2-NBDG

2-NBDG

== 2-NBDG ==

2-NBDG, or 2-Deoxy-2-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]-D-glucose, is a fluorescent analog of glucose commonly used in biological research to study glucose uptake and metabolism in cells. It is a non-metabolizable glucose analog that is taken up by cells through glucose transporters but cannot be further metabolized in the glycolytic pathway.

Structure

2-NBDG is structurally similar to glucose, with the exception of a nitrobenzoxadiazole moiety attached to the C-2 position of the glucose molecule. This modification allows 2-NBDG to emit fluorescence upon excitation, making it a valuable tool for visualizing glucose uptake in live cells.

Applications

2-NBDG is widely used in fluorescence microscopy and flow cytometry to monitor glucose uptake in various cell types. By tracking the uptake of 2-NBDG, researchers can gain insights into glucose transport kinetics, glucose utilization, and metabolic activity in different cell populations. In addition to its applications in basic research, 2-NBDG has also been utilized in studies related to cancer metabolism, diabetes, and other metabolic disorders. Its ability to specifically label glucose uptake provides a valuable tool for investigating the role of glucose metabolism in disease states.