ABI Solid Sequencing

ABI Solid Sequencing is a next-generation sequencing (NGS) technology developed by Applied Biosystems, which is now part of Thermo Fisher Scientific. This method is based on sequencing by ligation, a different approach from the more commonly known sequencing by synthesis used in other NGS platforms such as Illumina sequencing and Ion Torrent sequencing. ABI Solid Sequencing was introduced to the market to provide high-throughput sequencing solutions for genomic research.

Overview

ABI Solid Sequencing technology utilizes a unique method of DNA sequencing that involves the ligation of fluorescently labeled oligonucleotide probes to DNA templates. This process is cyclic and involves the sequential hybridization of probes, ligation, detection, and cleavage. Each cycle adds a known sequence of bases to the growing DNA strand, allowing for the determination of the DNA sequence based on the color-coded sequence of probes that have been ligated.

Technology

The core of ABI Solid Sequencing technology lies in its use of two-base encoding. In this system, each fluorescent dye is associated with a specific pair of bases rather than a single base. This method provides an inherent error-checking mechanism, as each base is read multiple times in different dinucleotide contexts. The sequencing process begins with the attachment of DNA fragments to a solid surface, followed by the ligation of fluorescently labeled probes that correspond to specific dinucleotide sequences. The identity of each probe is determined by its fluorescent label, and the sequence of the template DNA is deduced from the pattern of fluorescence observed over successive cycles of ligation and detection.

Applications

ABI Solid Sequencing has been applied in various areas of genomic research, including whole-genome sequencing, transcriptome analysis, and epigenomics. Its high-throughput capability and accuracy make it suitable for large-scale genomic studies. However, the complexity of data analysis and the specific advantages of other sequencing technologies have led to a diversification of sequencing technology choices among researchers.

Advantages and Disadvantages

One of the main advantages of ABI Solid Sequencing is its high accuracy, particularly in the context of repetitive sequences, due to the two-base encoding system. This feature reduces the impact of sequencing errors, which are a common challenge in NGS technologies. However, the technology also has its disadvantages, including a relatively complex library preparation and data analysis process. Additionally, the initial cost and operational complexity have limited its adoption compared to other NGS platforms.

Current Status

As of the last update, ABI Solid Sequencing technology has seen decreased usage in the face of rapid advancements and the introduction of newer, more efficient sequencing technologies. Thermo Fisher Scientific continues to support genomic research through the development of other sequencing platforms and technologies.

ABI Solid Sequencing Resources
Need Help Finding a Doctor? Need help finding a doctor or specialist anywhere in the world? Explore our world directory. WikiMD's DocFinder can assist you in locating millions of physicians.	Medical Knowledge Access the latest research - Most recent articles Ongoing trials.

Translate to: East Asian 中文, 日本, 한국어, South Asian हिन्दी, Urdu, বাংলা, తెలుగు, தமிழ், ಕನ್ನಡ,
Southeast Asian Indonesian, Vietnamese, Thai, မြန်မာဘာသာ, European español, Deutsch, français, русский, português do Brasil, Italian, polski