Fernbach flask

Fernbach flask

Uses	Culturing and growing microorganisms

The Fernbach flask is a type of laboratory glassware commonly used in microbiology and biotechnology for culturing and growing microorganisms. It is named after the American microbiologist H. Thomas Fernbach, who developed the design in the 1950s.

Design and Features

The Fernbach flask is a specialized flask with a unique shape that allows for efficient and large-scale microbial culture growth. It is typically made of borosilicate glass, which is resistant to thermal shock and chemical corrosion. The flask has a flat bottom and a wide, round body with a narrow neck and a sidearm for aeration and sampling.

The wide body of the Fernbach flask provides a large surface area for the growth of microorganisms. This design allows for increased oxygen transfer and better mixing of nutrients, resulting in improved cell growth and productivity. The flat bottom ensures stability during agitation or shaking, which is often required for optimal growth conditions.

Uses

The Fernbach flask is primarily used in laboratory settings for the cultivation of microorganisms, such as bacteria, yeast, and fungi. It is commonly employed in biotechnology, pharmaceutical, and research laboratories for various applications, including:

• Production of recombinant proteins: The Fernbach flask is often used for large-scale protein expression and purification. The increased surface area and improved oxygen transfer make it suitable for high-density cell cultures, resulting in higher protein yields.

• Fermentation studies: The flask is utilized for studying the fermentation process of microorganisms. It allows researchers to monitor and control various parameters, such as temperature, pH, and nutrient availability, to optimize the growth conditions and metabolic activities of the cultured organisms.

• Vaccine production: The Fernbach flask is employed in the production of vaccines, where large quantities of microorganisms need to be grown for antigen production. The flask's design facilitates the growth of high-density cultures, ensuring efficient vaccine production.

Maintenance and Sterilization

To ensure the success of microbial cultures, proper maintenance and sterilization of the Fernbach flask are crucial. Before use, the flask should be thoroughly cleaned and sterilized using autoclaving or other appropriate methods. It is important to handle the flask with care to avoid breakage or contamination.

See Also

• Laboratory glassware
• Microbiology
• Biotechnology