H&E stain
H&E stain or Hematoxylin and Eosin stain is a popular staining method in histology. It is named after the dyes hematoxylin and eosin, which it uses. Hematoxylin, which stains nuclei blue-purple, and eosin, which stains cytoplasm and the extracellular connective tissue matrix pink, are used.
Overview[edit | edit source]
H&E staining is used to examine tissues under a microscope and is one of the most commonly used stains in medical diagnosis. For example, it is often used to diagnose cancer. A pathologist will look at the tissue sample under a microscope and make a diagnosis based on the size, shape, and organization of the cells.
Procedure[edit | edit source]
The procedure for H&E staining is as follows:
- The tissue is fixed in a substance such as formalin to preserve it.
- The tissue is then cut into thin slices, or sections. These sections are placed on a glass slide.
- The slide is then stained with hematoxylin, which stains the cell nuclei blue.
- The slide is then stained with eosin, which stains the cytoplasm and connective tissue pink.
- The slide is then examined under a microscope.
Interpretation[edit | edit source]
The interpretation of H&E staining involves looking at the size, shape, and organization of the cells. Normal cells are uniform in size and shape, and are organized in a predictable pattern. Cancer cells, on the other hand, are often larger than normal cells, are irregular in shape, and are not organized in a predictable pattern.
See also[edit | edit source]
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