Immunophenotyping

From WikiMD's Food, Medicine & Wellness Encyclopedia

Pronounced IH-myoo-noh-FEE-noh-ty-ping, it is a process that uses antibodies to identify cells based on the types of antigens or markers on the surface of the cells.

Burkitt Lymphoma (FNA, Giemsa stain) (4679964756) On immunophenotyping by flow cytometry this FNA specimen was positive for CD10, CD20, and kappa sIg; negative for CD5 and lambda sIg.
Burkitt Lymphoma

Use in medicine[edit | edit source]

This process is used to diagnose specific types of leukemia and lymphoma by comparing the cancer cells to normal cells of the immune system.

Markers[edit | edit source]

Immunophenotyping may also be used to separate cells into different groups based on the markers they have on the surface.

Flow cytometry[edit | edit source]

It is a common flow cytometry test where fluorophore-conjugated antibodies are used as probes for staining target cells with high avidity and affinity.

Immunophenotyping Resources
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Contributors: Bonnu, Prab R. Tumpati, MD