Knockin

Knockin is a term used in genetics and molecular biology to refer to a process where a gene of interest is inserted at a specific locus in an organism's DNA. This process is used to study the function of the specific gene, and it is a powerful tool for creating model organisms that mimic human diseases.

Overview

The knockin technique is a form of genetic engineering that involves the insertion of a gene into a specific location in the genome. This is achieved through the use of homologous recombination, a process where DNA sequences that are similar or identical are exchanged between two DNA molecules.

In a knockin experiment, a vector containing the gene of interest is introduced into the cells of the organism. This vector also contains sequences that are homologous to the sequences flanking the desired insertion site in the genome. When the vector is introduced into the cells, the cellular machinery recognizes these homologous sequences and integrates the gene of interest at the desired location.

Applications

Knockin technology has a wide range of applications in biological research. It is often used to create model organisms that carry mutations associated with human diseases. These models can then be used to study the disease process and to test potential treatments.

Knockin is also used to study the function of specific genes. By inserting a gene at a specific location in the genome, researchers can control when and where the gene is expressed. This allows them to study the gene's function in a controlled manner.

See also

• Knockout (genetics)
• Gene targeting
• Transgenic organisms

References

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