Bacterial artificial chromosome

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A bacterial artificial chromosome (BAC) is an engineered DNA molecule used to clone DNA sequences in bacterial cells such as E. coli. {{Top}} may refer to:

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Use[edit | edit source]

BACs are often used in connection with DNA sequencing.

Segment length[edit | edit source]

  • Segments of an organism's DNA, ranging from 100,000 to about 300,000 base pairs, can be inserted into BACs.
  • The BACs, with their inserted DNA, are then taken up by bacterial cells.
  • As the bacterial cells grow and divide, they amplify the BAC DNA, which can then be isolated and used in sequencing DNA.

Frequently asked questions[edit | edit source]

Chromosome-DNA-gene copy
Chromosome-DNA-gene copy

Help answer these questions.

  • How do bacterial artificial chromosomes work?
  • How do you make a bacterial artificial chromosome?
  • How does a bacterial artificial chromosome BAC differ from a plasmid?
  • What must be present in artificial chromosome?
  • What are artificial chromosomes used for?
  • What is YAC and BAC?
  • What are BAC transgenic mice?
  • What is the F factor in bacteria?
  • Is YAC a plasmid?
  • What is BAC system?
  • What is an advantage of BACs and YACs over plasmids?
  • What is a BAC vector?
  • Who is YAC?
  • Is YAC a vector?
  • Is YAC circular or linear?
  • What are BACs and YACs How are they different?
  • What is the difference between plasmid and cosmid?
  • What do the sequence Tel signify in a YAC vector?
  • What is the cen4 region in the YAC vector?
  • What are cosmid vectors?
  • How can the presence of insert DNA in the YAC vector be checked?

External links[edit | edit source]

Wikipedia

Latest research[edit | edit source]

PubMed


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Contributors: Prab R. Tumpati, MD