T-47D

File:Karyotype of the T47D breast cancer cell line.svg

Karyotype_of_the_T47D_breast_cancer_cell_line.svg

== T-47D ==

T-47D is a human breast cancer cell line that is commonly used in scientific research. It was derived from the pleural effusion of a 54-year-old female patient with an infiltrating ductal carcinoma. T-47D cells are known for their hormone responsiveness, particularly to estrogen and progesterone, making them a valuable model for studying hormone receptor-positive breast cancer.

Characteristics

T-47D cells exhibit several key characteristics that make them useful for research:

• They express high levels of estrogen receptor (ER) and progesterone receptor (PR).
• They have a relatively stable karyotype.
• They are capable of forming tumors when injected into immunocompromised mice.

Applications in Research

T-47D cells are widely used in various areas of breast cancer research, including:

• Studying the mechanisms of hormone action and resistance.
• Testing the efficacy of anti-estrogen and anti-progesterone therapies.
• Investigating the role of specific genes and proteins in breast cancer progression.

Culturing T-47D Cells

T-47D cells are typically cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS) and 0.2 units/mL of insulin. They require a humidified atmosphere with 5% carbon dioxide (CO2) at 37°C.

See Also

• MCF-7
• Breast cancer cell lines
• Estrogen receptor
• Progesterone receptor
• Hormone therapy (oncology)