Trypan blu

Trypan Blue is a vital stain used in cell biology to selectively color dead tissues or cells blue. It is a diazo dye.

History[edit | edit source]

Trypan Blue was first synthesized by the German scientist Paul Ehrlich in 1904. It was named after the Trypanosoma genus of parasitic protozoa, which the dye was initially used to stain for microscopic examination.

Usage[edit | edit source]

In cell biology and microbiology, Trypan Blue is primarily used as a cell viability dye. Due to its large size and negative charge, Trypan Blue cannot pass through the cell membrane of healthy cells. However, it can penetrate and stain cells that have compromised membranes, such as dead or dying cells. This property makes it a useful tool for assessing cell viability in cell cultures.

In addition to its use in cell viability assays, Trypan Blue is also used in the staining of reticular fibers, myelin, and collagen in histology, and in the visualization of the anterior chamber of the eye in ophthalmology.

Safety[edit | edit source]

While Trypan Blue is generally considered safe for laboratory use, it can be harmful if ingested or inhaled, and can cause skin and eye irritation. Therefore, appropriate safety measures should be taken when handling this chemical.

See Also[edit | edit source]

This cell biology article is a stub. You can help WikiMD by expanding it.

Trypan blu Resources
Need Help Finding a Doctor? Need help finding a doctor or specialist anywhere in the world? Explore our world directory. WikiMD's DocFinder can assist you in locating millions of physicians.	Medical Knowledge Access the latest research - Most recent articles Ongoing trials.