Hexadimethrine bromide

Polybrene_with_bromide.svg

Hexadimethrine bromide is a chemical compound used primarily in the field of molecular biology and biochemistry. It is a polycationic substance that is often employed to enhance the efficiency of transfection processes, where foreign DNA is introduced into eukaryotic cells.

Chemical Properties

Hexadimethrine bromide is a polymer composed of repeating units of dimethrine, with bromide ions serving as counterions. Its polycationic nature allows it to interact with the negatively charged phosphate groups of nucleic acids, facilitating their entry into cells.

Applications

Transfection

One of the primary uses of hexadimethrine bromide is in the transfection of mammalian cells. It is often used in conjunction with other transfection agents to improve the uptake of plasmid DNA or siRNA into cells. The compound works by neutralizing the negative charges on the DNA, allowing it to pass through the cell membrane more easily.

Anticoagulant

Hexadimethrine bromide also has applications as an anticoagulant. It can be used to neutralize the effects of heparin, a common anticoagulant used during surgery and other medical procedures. This makes it useful in clinical settings where the reversal of heparin's effects is necessary.

Mechanism of Action

The polycationic nature of hexadimethrine bromide allows it to form complexes with negatively charged molecules such as DNA and RNA. This interaction facilitates the entry of these molecules into cells by reducing the electrostatic repulsion between the nucleic acids and the cell membrane.

Safety and Handling

Hexadimethrine bromide should be handled with care, as it can be toxic if ingested or inhaled. Proper laboratory safety protocols should be followed when working with this compound, including the use of personal protective equipment such as gloves and safety goggles.

See Also

• Transfection
• DNA
• RNA
• Heparin
• Anticoagulant

External Links

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